A list of some of the available 500wb MHz expts

• 90_times -- measured 90s for the 5mm TXI probe recently.

1D:

• macro: h1.txi -- 1D Proton -- pulprog
• macro: h1_es.txi -- 1D Proton with excitation sculpting water suppression -- pulprog
• macro: selnoe.txi -- 1D NOE Proton -- pulprog
• macro: selroe.txi -- 1D ROE Proton; used for MW of 700-1100 Da where NOEs don't show well. -- pulprog
  • cnst21 is the peak selected in ppm
  • using a 200ms mix time at 10kHz RF Field
• macro: c13.txi -- 1D 13C -- pulprog
• macro: f19.txi -- 1D 19F; need to wobb to 19F tuning using 1H tuners. -- pulprog
  • wobb using the "yellow" tuners
  • at zg/rga type in password "topspin" to make error go away
  • optional: set o1p near your peak if far away. This is the center of spectrum in ppm
• macro: std1d.txi -- STD Saturation Transfer Difference for ligand binding. -- ref1 -- macro_to_set_expt -- pulprog

2D:

• macro: kinetic_2d -- Series of 1D experiments for Kinetics (pseudo 2D file storage). See macro for defining delays etc. -- pulprog
• macro: h1_t2.txi -- Proton T2 measurement (pseudo 2D file storage). See macro for defining delays etc.
  • pulprog -- pulse program
  • mk_t2.pl -- perl script to make delays and loop files
  • t2_list -- t2 looping file in VC directory
  • t2_list.txt -- t2 delay values
• macro: noesy.txi -- 2D NOESY without water suppression. -- pulprog
• macro: cosy.txi -- 3 Pulse DQF COSY with gradient selection (organic compounds). Note it's magnitude mode. -- pulprog
• macro: clipcosy.txi -- Clip COSY - Clean in-phase COSY. Only see doublets (use this). -- ref1 -- pulprog
• macro: c_hsqc.txi -- H/C HSQC experiment. -- pulprog
• macro: c_h2bc.txi -- H/C 2-bond correlation experiment. -- pulprog -- ref
• macro: c_hmbc.txi -- H/C HMBC experiment. -- pulprog
• macro: dosy.txi -- DOSY without water suppression. -- pulprog

2D in Water:

• macro: cosy_es.txi -- COSY with water suppression. -- pulprog
• macro: noesy_es.txi -- NOESY with water suppression. -- pulprog
• macro: roesy_es.txi -- ROESY with water suppression. Use for case where we can't see NOESY due to molecular size. -- pulprog
• macro: tocsy_es.txi -- TOCSY with water suppression. -- pulprog
• macro: dosy_es.txi -- DOSY with 3-9-19 water suppression. -- pulprog
• macro: ipcosy.txi -- In-Phase COSY with water suppression. 3J from HN/HA correlations. -- ref1 -- pulprog



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